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Home >> Antibodies >> Spectrin Alpha 1 (Erythrocyte Marker)

Spectrin Alpha 1 (Erythrocyte Marker)

Mouse Monoclonal Antibody [Clone SPTA1/1832]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).

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Formalin-fixed, paraffin-embedded human Tonsil stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).

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SDS-PAGE Analysis Purified Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of Human K562 cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).

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Western Blot Analysis of Human HeLa cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin, alpha 1 Mouse Monoclonal Antibody (SPTA1/1832)

Product Details

Synonyms

Alpha I spectrin; Elliptocytosis 2 (EL2); Erythrocyte alpha spectrin; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha erythrocytic 1; SPH3; SPTA1

Positive Control

K562; HeLa; Jurkat cells. Placenta or Tonsil.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic and Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

240kDa

Specificity & Comments

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Speicher, D.W. 1986. J. Cell. Biochem. 30: 245-258.

Bioinformatics

  •  Entrez Gene ID: 6708
  •  Gene Symbol: SPTA1
  •  SwissProt: P02549
  •  Chromosome Location: 1q23.1
  •  Unigene: 119825
NBT01832