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Home >> Antibodies >> Spectrin Alpha 1 (Erythrocyte Marker)

Spectrin Alpha 1 (Erythrocyte Marker)

Recombinant Mouse Monoclonal Antibody [Clone rSPTA1/1832]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

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SDS-PAGE Analysis Purified Spectrin alpha 1 Mouse Recombinant Monoclonal (rSPTA1/1832). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human K562 cell lysate using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

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Western Blot Analysis of human HeLa cell lysate using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

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Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Product Details

Synonyms

Alpha I spectrin; Elliptocytosis 2 (EL2); Erythrocyte alpha spectrin; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha erythrocytic 1; SPH3; SPTA1

Positive Control

K562. HeLa, Jurkat cells. Placenta or Tonsil.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic and Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

240kDa

Specificity & Comments

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Speicher, D.W. 1986. J. Cell. Biochem. 30: 245-258.

Bioinformatics

  •  Entrez Gene ID: 6708
  •  Gene Symbol: SPTA1
  •  SwissProt: P02549
  •  Chromosome Location: 1q23.1
  •  Unigene: 119825
NBT02556