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S100A1 (Melanoma Marker) Antibody [S100A1/6374R]

In Stock
Catalog Number Formulation Size Price
6271-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6271-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6271-RBM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS PAGE Analysis Purified S100A1 Rabbit Monoclonal Antibody (S100A1/6374R) Confirmation of Purity and Integrity of Antibody.

SDS PAGE Analysis Purified S100A1 Rabbit Monoclonal Antibody (S100A1/6374R) Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Rabbit
Applications
Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Infectious Disease
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
Gene Name
S100A1
Positive Control
A431 or HT-29 cells. Human brain or melanoma., T98G
Immunogen
Purified human S-100 protein conjugated to methylated BSA
Alternate Names
Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1, Bpb; NEF; S100-A1; S100 Alpha Chain; S100 Beta Chain; S100 Calcium Binding Protein A1; S100 Calcium Binding Protein B; S100 Calcium Binding Protein Beta Neural

Database Links

Entrez Gene ID
6271
SwissProt
P23297

Additional Information

Clone
S100A1/6374R
Chromosome Location
1q21.3
Mol. Weight of Antigen
10-12kDa

Related Antibodies

View All S100A1 Antibodies

Functions

  • Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).

Key References

  • Vanstapel et al. 1986. Am J Clin Pathol. 85(2):160-8.
  • Vanstapel et al. 1985. Lab Invest. 52(2):232-8.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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