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Home >> Antibodies >> Replication Protein A2 (RPA2)

Replication Protein A2 (RPA2)

Mouse Monoclonal Antibody [Clone RPA2/2106]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Western Blot Analysis of Human A375, K562, MCF-7 cell lysate using Replication Protein A2 Mouse Monoclonal Antibody (RPA2/2106).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Replication Protein A2 Mouse Monoclonal Antibody (RPA2/2106).

Product Details

Synonyms

p32; p34; REPA2; Replication protein A 32kDa subunit; Replication protein A 34kDa subunit; Replication protein A2; RF-A protein 2; Rf-A2; RFA; RP-A p32; RP-A p34; RP21C; RPA32; RPA34; RpLP1; RpP2

Positive Control

MCF-7, HeLa or HepG2 cells. Human tonsil (IHC).

Known Applications & Suggested Dilutions

Western Blot         More Details

Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length Replication protein A2 (RPA2) protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

32-34kDa

Specificity & Comments

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kenny MK, et. al. Journal of Biological Chemistry, 1990, 265(13):7693-7700.

Bioinformatics

  •  Entrez Gene ID: 6118
  •  Gene Symbol: RPA2
  •  SwissProt: P15927
  •  Chromosome Location: 1p35.3
  •  Unigene: 703070, 79411
NBT02106