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Home >> Antibodies >> PU.1 (SPI-1) (B-Cell Marker)

PU.1 (SPI-1) (B-Cell Marker)

Mouse Monoclonal Antibody [Clone PU1/2146]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).

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Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).

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Formalin-fixed, paraffin-embedded human Spleen stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).

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Western Blot Analysis of THP-1 cell lysate using PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).

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SDS-PAGE Analysis Purified PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1 Mouse Monoclonal Antibody (PU1/2146) followed by goat anti-mouse IgG-CF488 (Green). Membrane is labeled with Phalloiden (Red).

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Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1-Monospecific Mouse MAb (PU1/2146); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2446).

Product Details

Synonyms

Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Positive Control

Ramos, K562 or THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, Immunohistology         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Loddenkemper C, et al. J Pathol. 2004; 202:60-9.

Bioinformatics

  •  Entrez Gene ID: 6688
  •  Gene Symbol: SPI1
  •  SwissProt: P17947
  •  Chromosome Location: 11p11.2
  •  Unigene: 502511