2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> PMS1

PMS1

Mouse Monoclonal Antibody [Clone PCRP-PMS1-2E11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

SDS-PAGE Analysis Purified PMS1 Mouse Monoclonal Antibody (PCRP-PMS1-2E11). Confirmation of Purity and Integrity of Antibody.

img

Flow cytometric analysis of PFA-fixed HeLa cells. PMS1 Mouse Monoclonal Antibody (PCRP-PMS1-2E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PMS1 Mouse Monoclonal Antibody (PCRP-PMS1-2E11) [CF488]. PMS1 localized to nucleoplasm and nuclear bodies. Microtubules stained with CF640R.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using PMS1-Monospecific Mouse Monoclonal Antibody (PCRP-PMS1-2E11).

Product Details

Synonyms

DNA mismatch repair protein PMS1; HNPCC3; hPMS1; Human homolog of yeast mutL; PMS1 postmeiotic segregation increased 1 (S. cerevisiae); PMS1 protein homolog 1; PMSL1; Rhabdomyosarcoma antigen MU RMS 40.10B / 40.10E

Positive Control

HeLa, U-251 MG or CACO-2 cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF         More Details

ELISA (concentration is assay dependent)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PMS1 protein

Cellular Localization

Nucleus. Nuclear bodies.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

105.8kDa

Specificity & Comments

The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression. Two additional homologs of the prokaryotic MutL gene, designated PMS1 and PMS2, have been identified and shown to be mutated in the germline of HNPCC patients.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 5378
  •  Gene Symbol: PMS1
  •  SwissProt: P54277
  •  Chromosome Location: 2q32.2
  •  Unigene: 111749