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Home >> Antibodies >> PLK1 (Marker of Mitosis)

PLK1 (Marker of Mitosis)

Mouse Monoclonal Antibody [Clone AZ44]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified PLK1 Mouse Monoclonal Antibody (AZ44). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Cell cycle regulated protein kinase; Polo like kinase 1 (PLK1); Serine/threonine-protein kinase PLK1; STPK13

Positive Control

Mitotic CSF Xenopus egg extract.

Known Applications & Suggested Dilutions

ELISA, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length PLK1 Xenopus laevis protein

Cellular Localization

Nucleus and cytoplasm.

Species Reactivity

Xenopus laevis. Does not react with Human or Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

66kDa

Specificity & Comments

Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle; Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sunkel, C.E. and Glover, D.M. 1988. J. Cell Sci. 89: 25-38.

Bioinformatics

  •  Entrez Gene ID: 380481
  •  Gene Symbol: plk1.S
  •  SwissProt: P70032
  •  Chromosome Location: 9_10S (Xenopus laevis)
  •  Unigene: 28141
NBT03101