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Home >> Antibodies >> PGP9.5 / UchL1

PGP9.5 / UchL1

Mouse Monoclonal Antibody [Clone SPM574]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (SPM574).

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Western Blot Analysis of human Brain lysate using Pgp9.5 / UchL1 Monoclonal Antibody (SPM574).

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Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (SPM574) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)

Product Details

Synonyms

Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1

Positive Control

T98G cells. Cerebellum.

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Native UchL1 (PGP9.5) protein from brain

Cellular Localization

Cytoplasmic. Endoplasmic Reticulum membrane

Species Reactivity

Human. Mouse. Rat. Cow. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

20-30kDa

Specificity & Comments

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Day IN et. al. Biochem Society Trans 14:350-351 (1986)

Bioinformatics

  •  Entrez Gene ID: 7345
  •  Gene Symbol: UCHL1
  •  SwissProt: P09936
  •  Chromosome Location: 4p13
  •  Unigene: 518731
NBT00046