Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (SPM574).
Western Blot Analysis of human Brain lysate using Pgp9.5 / UchL1 Monoclonal Antibody (SPM574).
Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (SPM574) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.