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Home >> Antibodies >> Perforin-1 (Pore Forming Protein) (Apoptosis Marker)

Perforin-1 (Pore Forming Protein) (Apoptosis Marker)

Mouse Monoclonal Antibody [Clone PRF1/2470]

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Catalog No Formulation Size Price (USD)  
5551-MSM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
5551-MSM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
5551-MSM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 5551
Human SwissProt P14222
Human Unigene 2200
Human Gene Symbol PRF1
Human Chromosome Location 10q22.1
Synonyms Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF
Immunogen Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2c, kappa
Mol. Weight of Antigen 75kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others not known.

Specificity & Comments

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

Known Applications & Suggested Dilutions

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HDLM-2 Cells. Spleen

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Bittmann I, et al. Fas/FasL and perforin/granzyme pathway in acute rejection and diffuse alveolar damage after allogeneic lung transplantation- a human biopsy study. Virchows Arch. 2004; 445:375-81.
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