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Home >> Antibodies >> Perforin-1 (Pore Forming Protein) (Apoptosis Marker)
Perforin-1 (Pore Forming Protein) (Apoptosis Marker)

Mouse Monoclonal Antibody [Clone PRF1/2470]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Mouse Monoclonal Antibody (PRF1/2470).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Perforin-1 Mouse Monoclonal Antibody (PRF1/2470).

Product Details

Synonyms

Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF

Positive Control

HDLM-2 cells. Spleen

Known Applications & Suggested Dilutions

IHC-P         More Details
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG2c, kappa

Specificity & Comments

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bittmann I, et al. Fas/FasL and perforin/granzyme pathway in acute rejection and diffuse alveolar damage after allogeneic lung transplantation- a human biopsy study. Virchows Arch. 2004; 445:375-81.

Bioinformatics

  •  Entrez Gene ID: 5551
  •  Gene Symbol: PRF1
  •  SwissProt: P14222
  •  Chromosome Location: 10q22.1
  •  Unigene: 2200
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