|Catalog No||Formulation||Size||Price (USD)|
|5551-MSM4-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|5551-MSM4-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||429.00|
|5551-MSM4-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||429.00|
|Human Entrez Gene ID||5551|
|Human Gene Symbol||PRF1|
|Human Chromosome Location||10q22.1|
|Synonyms||Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF|
|Immunogen||Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG2c, kappa|
|Mol. Weight of Antigen||75kDa|
|Species Reactivity||Human. Others not known.|
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HDLM-2 Cells. Spleen
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.