Western Blot Analysis of Raji, HepG2, THP-1, RAW, NIH3T3, U87 cell lysates using PD-L2 Mouse Monoclonal Antibody (PDL2/2676).
Flow Cytometric Analysis of Jurkat cells using PD-L2 Mouse Monoclonal Antibody (PDL2/2676) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence Analysis of Jurkat cells labeling PD-L2 with PD-L2 Mouse Monoclonal Antibody (PDL2/2676) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
SDS-PAGE Analysis Purified PD-L2 Mouse Monoclonal Antibody (PDL2/2676). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L2 Mouse Monoclonal Antibody (PDL2/2676)
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of about 31kDa, which is identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.