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Home >> Antibodies >> PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

Mouse Monoclonal Antibody [Clone PDL1/2746]

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Catalog No Formulation Size Price (USD)  
29126-MSM6-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
29126-MSM6-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
29126-MSM6-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug
Human Entrez Gene ID 29126
Human SwissProt Q9NZQ7
Human Unigene 521989
Human Gene Symbol CD274
Human Chromosome Location 9p24.1
Synonyms B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1
Immunogen Recombinant fragment of human CD274 protein (around aa 39-191) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 37-50kDa
Cellular Localization Cell Surface and Cytoplasmic
Species Reactivity Human and Mouse. Others not known.

Specificity & Comments

Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. Engagement of CTLA4 by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acid type I transmembrane protein, which is 20% and 15% identical to B7-1 and B7-2, respectively, has immunoglobulin V-like and C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2 protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L system could provide unique negative signaling to help prevent autoimmune diseases.

Known Applications & Suggested Dilutions

ELISA (For coating, order antibody without BSA)
Western Blotting (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Positive Control

Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat Cells.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Dong, H., et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat. Med. 5: 1365-1369
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