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Home >> Antibodies >> PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)
PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

Mouse Monoclonal Antibody [Clone PDL1/2746]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Lung SCC stained with PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of HEK293 and HepG2 cell lysates using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Western Blot Analysis of RAW, A549, THP1, MOLT-4, Raji, and MDA-MB-231 cell lysates using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Flow Cytometric Analysis of human Jurkat cells using PD-L1 Mouse Monoclonal Antibody (PDL1/2746) followed by Goat anti-mouse IgG-CF488 (Orange) ; Cells alone (Blue) ; Isotype Control (Red).

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Immunofluorescence Analysis of Jurkat cells labeling PD-L1 with PD-L1 Mouse Monoclonal Antibody (PDL1/2746) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

Product Details

Synonyms

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Positive Control

Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat cells.

Known Applications & Suggested Dilutions

ELISA, WB, Flow Cyt, IF, IHC-P         More Details
ELISA (For coating, order antibody without BSA)
Western Blotting (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD274 protein (around aa 39-191) (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Mouse. Others not known.

Host / Ig Isotype

Mouse / IgG2b, kappa

Specificity & Comments

Engagement of by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. Engagement of CTLA4 by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acid type I transmembrane protein, which is 20% and 15% identical to B7-1 and B7-2, respectively, has immunoglobulin V-like and C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2 protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L system could provide unique negative signaling to help prevent autoimmune diseases.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Dong, H., et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat. Med. 5: 1365-136

Bioinformatics

  •  Entrez Gene ID: 29126
  •  Gene Symbol: CD274
  •  SwissProt: Q9NZQ7
  •  Chromosome Location: 9p24.1
  •  Unigene: 521989
DOC#02746