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Home >> Antibodies >> PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

Mouse Monoclonal Antibody [Clone PDL1/2746]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Lung SCC stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746).

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Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746).

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Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746).

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SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of HEK293 and HepG2 cell lysates using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Western Blot Analysis of RAW, A549, THP1, MOLT-4, Raji, and MDA-MB-231 cell lysates using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

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Flow Cytometric Analysis of human Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).

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Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746).

Product Details

Synonyms

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Positive Control

Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat cells.

Known Applications & Suggested Dilutions

ELISA, Western Blot, Flow Cyt, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

37-50kDa

Specificity & Comments

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Dong, H., et al. 1999. Nat. Med. 5: 1365-1369.

Bioinformatics

  •  Entrez Gene ID: 29126
  •  Gene Symbol: CD274
  •  SwissProt: Q9NZQ7
  •  Chromosome Location: 9p24.1
  •  Unigene: 521989
NBT02746