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Home >> Antibodies >> PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

Mouse Monoclonal Antibody [Clone PDL1/2744]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of HeLa, Raji, MDA231, MCF-7, NIH3T3, Panc-1, cell lysates using PD-L2 Mouse Monoclonal Antibody (PDL1/2744).

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SDS-PAGE Analysis Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2744). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1 Mouse Monoclonal Antibody (PDL1/2744).

Product Details

Synonyms

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Positive Control

Jurkat, HEK293 or HepG2 cells. Lung SqCC, Cervical or Breast Carcinoma.

Known Applications & Suggested Dilutions

ELISA, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD274 protein (around aa39-191) (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

37-50kDa

Specificity & Comments

Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. Engagement of CTLA4 by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acid type I transmembrane protein, which is 20% and 15% identical to B7-1 and B7-2, respectively, has immunoglobulin V-like and C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2 protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L system could provide unique negative signaling to help prevent autoimmune diseases.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Dong, H., et al. 1999. Nat. Med. 5: 1365-1369.

Bioinformatics

  •  Entrez Gene ID: 29126
  •  Gene Symbol: CD274
  •  SwissProt: Q9NZQ7
  •  Chromosome Location: 9p24.1
  •  Unigene: 521989
NBT02744