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Home >> Antibodies >> PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)

Mouse Monoclonal Antibody [Clone PCNA/694]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of HepG2 cell lysate using PCNA Monoclonal Antibody (PCNA/694)

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Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)

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Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Monoclonal Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

Positive Control

Tonsil or reactive lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full length human PCNA protein

Cellular Localization

Predominantly nuclear, some cytoplasmic

Species Reactivity

Human. Shows broad species reactivity.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Waseem NH Lane DP. 1990. J Cell Sci. 96:121-9.
  2. Hall PA et al. 1990. J. Pathol. 162(4):285-94.

Bioinformatics

  •  Entrez Gene ID: 5111
  •  Gene Symbol: PCNA
  •  SwissProt: P12004
  •  Chromosome Location: 20p12.3
  •  Unigene: 147433, 728886
NBT00694