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Home >> Antibodies >> p57Kip2 (Mitotic Inhibitor/Suppressor Protein)

p57Kip2 (Mitotic Inhibitor/Suppressor Protein)

Mouse Monoclonal Antibody [Clone KIP2/880]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p57 Mouse Monoclonal Antibody (KIP2/880).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p57 Mouse Monoclonal Antibody (KIP2/880).

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SDS-PAGE Analysis p57 Mouse Monoclonal Antibody (KIP2/880). Confirmation of Purity and Integrity of Antibody

Product Details

Synonyms

Beckwith Wiedemann syndrome (WBS); BWCR; Cyclin dependent kinase inhibitor 1C (CDKN1C); Cyclin dependent kinase inhibitor p57; KIP2; p57; p57Kip2

Positive Control

LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human p57Kip2 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

57kDa

Specificity & Comments

Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lee, M.-H., et al. 1995. Cloning of p57, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 9: 639-649.

Bioinformatics

  •  Entrez Gene ID: 1028
  •  Gene Symbol: CDKN1C
  •  SwissProt: P49918
  •  Chromosome Location: 11p15.5
  •  Unigene: 106070
NBT00880