Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p53 Mouse Monoclonal Antibody (TP53/1739).
Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (TP53/1739).
SDS-PAGE Analysis Purified p53 Mouse Monoclonal Antibody (TP53/1739). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using p53 Mouse Monoclonal Antibody (TP53/1739).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. It is a tumor suppressor protein expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast, lung, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in many malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.