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Home >> Antibodies >> p53 Tumor Suppressor Protein

p53 Tumor Suppressor Protein

Recombinant Mouse Monoclonal Antibody [Clone rTP53/1739]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739)

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SDS-PAGE Analysis Purified p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of HeLa cell lysate using p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using p53 Mouse Monoclonal Antibody (rTP53/1739).

Product Details

Synonyms

Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Positive Control

MDA-MB-231 cells. Breast or Colon carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human TP53 protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

53kDa

Specificity & Comments

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. It is a tumor suppressor protein expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast, lung, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in many malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Vojtesek B et al. 1992. J. Immunol. Methods. 151(1-2): 237-44.
  2. Stephen CW et al. 1995. J Mol. Biol. 248(1): 58-78.

Bioinformatics

  •  Entrez Gene ID: 7157
  •  Gene Symbol: TP53
  •  SwissProt: P04637
  •  Chromosome Location: 17p13.1
  •  Unigene: 654481
NBT02091