2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> p27Kip1 (Mitotic Inhibitor/Suppressor Protein)

p27Kip1 (Mitotic Inhibitor/Suppressor Protein)

Mouse Monoclonal Antibody [Clone KIP1/769]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

429.00
img

Flow Cytometry of human p27 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled p27 Monoclonal Antibody (KIP1/769).

Product Details

Synonyms

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Positive Control

ZR75, T47D, SK-BR-3, MDA-MB-231, MCF7 cells. Tonsil, Breast or Colon Ca.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human KIP1 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat. Monkey.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

25-26kDa

Specificity & Comments

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Related Products



Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, et al. High level expression of p27Kip1and cyclin D1 in some human breast cancer cells: Inverse correlation between the expression of p27Kip1 and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci 1997;94:6380-5.

Bioinformatics

  •  Entrez Gene ID: 1027
  •  Gene Symbol: CDKN1B
  •  SwissProt: P46527
  •  Chromosome Location: 12p13.1
  •  Unigene: 238990
NBT00769