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Home >> Antibodies >> P16INK4a

P16INK4a

Recombinant Rabbit Monoclonal Antibody [Clone CDKN2A/8223R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/8223R). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/8223R). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

img

SDS-PAGE Analysis Purified p16 Recombinant Rabbit Monoclonal Antibody (CDKN2A/8223R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CDK4 inhibitor p16 INK4; CDK4I; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent kinase 4 inhibitor A; Melanoma p16 inhibits CDK4; MLM; MTS1; Multiple tumor suppressor 1; p14; p16; p19; P19ARF; TP16

Positive Control

Human cervical squamous cell carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Purified recombinant prokaryotic full-length human p16INK4a protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

14kDa

Specificity & Comments

p16INK4a is a tumor suppressor protein. It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16INK4a abnormalities is higher in tumorā€derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia. Expression of p16INK4a (p16 positive) is highly correlated with human papilloma virus (HPV) infection in head and neck squamous cell carcinomas (HNSCC). p16 status is an important prognostic indicator in HNSCC and the p16 positive/HPV16 negative group is likely a distinct subgroup lacking any HPV genotype.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Mahajan A. Hum Pathol. 2016;51:64€74.
  2. Hunter, T. 1993. Braking the cycle. Cell 75: 839-841.

Bioinformatics

  •  Entrez Gene ID: 1029
  •  Gene Symbol: CDKN2A
  •  SwissProt: P42771
  •  Chromosome Location: 9p21.3
  •  Unigene: 512599