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Home >> Antibodies >> OCT-2 (POU2F2) (B-Cell Marker)

OCT-2 (POU2F2) (B-Cell Marker)

Mouse Monoclonal Antibody [Clone OCT2/2136]

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Catalog No Formulation Size Price (USD)  
5452-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
5452-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
5452-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 5452
Human SwissProt P09086
Human Unigene 147381
Human Gene Symbol POU2F2
Human Chromosome Location 19q13.2
Synonyms Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; POU domain class 2 transcription factor 2; POU2F2
Immunogen Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 60kDa
Cellular Localization Nuclear
Species Reactivity Human. Others not known.

Specificity & Comments

Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. Oct-2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of Oct-2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of Oct-2. Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for Oct-2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.

Known Applications & Suggested Dilutions

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Raji or U-937 cells. Spleen or Tonsil.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Gibson SE, et al. Am J Clin Pathol. 2006; 126:916-24.
  2. Browne P, et al. Am J Clin Pathol. 2003; 120:767-77.
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