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Home >> Antibodies >> Nucleophosmin (Acute Myeloid Leukemia Marker)

Nucleophosmin (Acute Myeloid Leukemia Marker)

Mouse Monoclonal Antibody [Clone NPM1/3398]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Nucleophosmin Mouse Monoclonal Antibody (NPM1/3398).

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Formalin-fixed, paraffin-embedded human ColonCarcinoma stained with Nucleophosmin Mouse Monoclonal Antibody (NPM1/3398).

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Purified Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3398). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Nucleophosmin Mouse Monoclonal Antibody (3398). Z- and S-

Product Details

Synonyms

NO38; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; Nucleophosmin/nucleoplasmin family member 1; Numatrin

Positive Control

MCF-7, HeLa or A431 cells. Skin, Colon.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)

Cellular Localization

Nuclear and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

33kDa

Specificity & Comments

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm.It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Chou, Y.H., et al. 1995. Cell cycle phase-dependent changes of localization and oligomerization states of nucleophosmin/B23. Biochem. Biophys. Res. Commun. 217: 313-325.
  2. Nozawa, Y., et al. 1996. Expression of nucleophosmin/B23 in normal and neoplastic colorectal mucosa. J. Pathol. 178: 48-52.

Bioinformatics

  •  Entrez Gene ID: 4869
  •  Gene Symbol: NPM1
  •  SwissProt: P06748
  •  Chromosome Location: 5q35.1
  •  Unigene: 557550
NBT03398