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Home >> Antibodies >> NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)

NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)

Mouse Monoclonal Antibody [Clone ENO2/4507]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human pancreas stained with NSE gamma Mouse Monoclonal Antibody (ENO2/4507).

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Formalin-fixed, paraffin-embedded human pancreas stained with NSE gamma Mouse Monoclonal Antibody (ENO2/4507).

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Western blot analysis of A431 cell lysate using NSE gamma Mouse Monoclonal Antibody (ENO2/4507).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to NSE gamma (ENO2/4507).

Product Details

Synonyms

2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE

Positive Control

Jurkat cells. Human pancreas, cerebellum or pheochromocytoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human NSE gamma (around aa416-433) (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

~50kDa

Specificity & Comments

This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.  It it be usually employed in combination with other markers such as Synaptophysin,  Chromogranin A, and Neurofilament.  

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.

Bioinformatics

  •  Entrez Gene ID: 2026
  •  Gene Symbol: ENO2
  •  SwissProt: P09104
  •  Chromosome Location: 12p13
  •  Unigene: 511915