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Home >> Antibodies >> NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)

NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)

Mouse Monoclonal Antibody [Clone ENO2/1375]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).

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Formalin-fixed, paraffin-embedded human Cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).

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Western Blot of Y79, HeLa and HepG2 cell lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).

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Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Mouse Monoclonal Antibody (ENO2/1375).

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Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Mouse Monoclonal Antibody (ENO2/1375).

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SDS-PAGE Analysis Purified NSE gamma Mouse Monoclonal Antibody (ENO2/1375). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE

Positive Control

HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.2-0.4ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

~50kDa

Specificity & Comments

Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.

Bioinformatics

  •  Entrez Gene ID: 2026
  •  Gene Symbol: ENO2
  •  SwissProt: P09104
  •  Chromosome Location: 12p13
  •  Unigene: 511915
NBT01375