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NRF1

Mouse Monoclonal Antibody [Clone NRF1/2609]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

429.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified NRF1 Mouse Monoclonal Antibody (NRF1/2609). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using NRF1 Mouse Monoclonal Antibody (NRF1/2609).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with NRF1 Mouse Monoclonal Antibody (NRF1/2609).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with NRF1 Mouse Monoclonal Antibody (NRF1/2609).

SDS-PAGE Analysis Purified NRF1 Mouse Monoclonal Antibody (NRF1/2609). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using NRF1 Mouse Monoclonal Antibody (NRF1/2609).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with NRF1 Mouse Monoclonal Antibody (NRF1/2609).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with NRF1 Mouse Monoclonal Antibody (NRF1/2609).

Product Details

Synonyms

alpha pal; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; Nuclear respiratory factor 1 (NFR-1)

Positive Control

Ubiquitous expression, strongest in skeletal muscle.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human NRF1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

30kDa (bZIP region); 65-120kDa (glycosylated)

Specificity & Comments

The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.