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Home >> Antibodies >> NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)

NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)

Mouse Monoclonal Antibody [Clone NGFR/4919]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human melanoma stained with NGFR Mouse Monoclonal Antibody (NGFR/4919) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human melanoma stained with NGFR Mouse Monoclonal Antibody (NGFR/4919) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

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SDS-PAGE Analysis Purified NGFR Mouse Monoclonal Antibody (NGFR/4919). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using NGFR-Monospecific Mouse Monoclonal Antibody (NGFR/4919).

Product Details

Synonyms

CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; p75 ICD; p75 Neurotrophin receptor; Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)

Positive Control

Neuronal axons, Schwann cells, perineural cells of peripheral nerves, tumors of nerve sheath differentiation. Melanomas.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

WM245 melanoma cells of human origin.

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human. Expected reactivity in mouse and rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

75kDa

Specificity & Comments

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Soland, T.M., et al. 2008. Histopathology 53: 62-72.
  2. Wang, W., et al. 2009. Eur. J. Surg. Oncol. 35: 826-832.

Bioinformatics