2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Neurofilament (NF-L) (Neuronal Marker)

Neurofilament (NF-L) (Neuronal Marker)

Mouse Monoclonal Antibody [Clone NR-4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NR-4).

img

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NR-4).

img

SDS-PAGE Analysis Purified Neurofilament Mouse Monoclonal Antibody (NR-4). Confirmation of Integrity and Purity of Antibody

img

Flow Cytometric Analysis of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

img

Immunofluorescence staining of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

Product Details

Synonyms

68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68

Positive Control

HEK293 cells; Brain, Neuroblastoma.

Known Applications & Suggested Dilutions

IF, Flow Cyt, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Crude neurofilament preparation from porcine spinal cord

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Rat. Pig. Cow. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

68kDa

Specificity & Comments

This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Debus E., Weber K., Osborn M. Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides. Differentiation 25 (2): 193-203, (1983).
  2. Angelides, K.J., et. al. 1989. J. Cell Biol. 108: 1495-1506.

Bioinformatics

  •  Entrez Gene ID: 4747
  •  Gene Symbol: NEFL
  •  SwissProt: P07196
  •  Chromosome Location: 8p21.2
  •  Unigene: 521461
NBT00303