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Home >> Antibodies >> Nestin (Cancer Stem Cell Marker)

Nestin (Cancer Stem Cell Marker)

Mouse Monoclonal Antibody [Clone NES/2911]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nestin Mouse Monoclonal Antibody (NES/2911).

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Formalin-fixed, paraffin-embedded human Kidney stained with Nestin Mouse Monoclonal Antibody (NES/2911).

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Formalin-fixed, paraffin-embedded human Kidney stained with Nestin Mouse Monoclonal Antibody (NES/2911).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nestin Mouse Monoclonal Antibody (NES/2911).

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SDS-PAGE Analysis Purified Nestin Mouse Monoclonal Antibody (NES/2911). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ESTM 46; FLJ21841; Intermediate filament protein; Nbla00170; nes

Positive Control

Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human NES protein fragment around aa 698-844 (exact sequence is proprietary)

Cellular Localization

Cytoplasm and membrane.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG

Mol. Weight of Antigen

190-200kDa

Specificity & Comments

Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zhang Y, et al. Nat Commun. 2018 Sep 6;9(1):3613
  2. Yamahatsu, K., et al. (2012). International Journal of Oncology, 40, 1345-1357.

Bioinformatics

  •  Entrez Gene ID: 10763
  •  Gene Symbol: NES
  •  SwissProt: P48681
  •  Chromosome Location: 1q23.1
  •  Unigene: 527971
NBT02911