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Home >> Antibodies >> Napsin A (Lung Adenocarcinoma Marker)

Napsin A (Lung Adenocarcinoma Marker)

Mouse Monoclonal Antibody [Clone NAPSA/1238 + NAPSA/1239]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238 + NAPSA/1239).

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Western Blot of K562, HEK293 andA549 cell lysates Using Napsin-A Monoclonal Antibody (NAPSA/1238 + NAPSA/1239)

Product Details

Synonyms

ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Positive Control

Lung adenocarcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG, kappa

Mol. Weight of Antigen

37kDa

Specificity & Comments

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

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200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Ordonez, NG 2012 Adv Anat Pathol 19: 66-73
  2. Bishop, JA et. al. Hum Pathol 41: 20-25
  3. Ye, J et. al. Appl Immunhistochem Mol Morphol 19: 313-317

Bioinformatics

  •  Entrez Gene ID: 9476
  •  Gene Symbol: NAPSA
  •  SwissProt: O96009
  •  Chromosome Location: 19q13.33
  •  Unigene: 512843
NBT01254