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Home >> Antibodies >> Myogenin / Myf-4 (Skeletal Muscle Marker)

Myogenin / Myf-4 (Skeletal Muscle Marker)

Mouse Monoclonal Antibody [Clone F5D]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin Mouse Monoclonal Antibody (F5D)

Product Details

Synonyms

bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Positive Control

Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (For coating, order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat. Dog. Cat. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 1996; 19:31

Bioinformatics

  •  Entrez Gene ID: 4656
  •  Gene Symbol: MYOG
  •  SwissProt: P15173
  •  Chromosome Location: 1q32.1
  •  Unigene: 2830
NBT00632