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Home >> Antibodies >> MyoD1 (Rhabdomyosarcoma Marker)

MyoD1 (Rhabdomyosarcoma Marker)

Recombinant Mouse Monoclonal Antibody [Clone rMYD712]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712).

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SDS-PAGE Analysis Purified MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712).

Product Details

Synonyms

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Positive Control

Rhabdomyosarcoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MyoD1 protein

Cellular Localization

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.
  2. Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
  3. Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11.

Bioinformatics

  •  Entrez Gene ID: 4654
  •  Gene Symbol: MYOD1
  •  SwissProt: P15172
  •  Chromosome Location: 11p15.1
  •  Unigene: 181768
NBT02329