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Myelin Basic Protein

Mouse Monoclonal Antibody [Clone MBP/4272]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

SDS-PAGE Analysis Purified Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of U87 cell lysate using Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

Western blot analysis of human brain tissue lysate using Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

SDS-PAGE Analysis Purified Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of U87 cell lysate using Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

Western blot analysis of human brain tissue lysate using Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4272).

Product Details

Synonyms

GDB; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MLD; Myelin A1 Protein, basic; Myelin Deficient; Myelin membrane encephalitogenic protein; SHI; Shiverer; SP

Positive Control

U87 cells. Human brain or astrocytoma tissue.

Known Applications & Suggested Dilutions

ELISA, IF, Western Blot, IHC-P More Details

ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recognizes bovine BMP, in the region of residues 119-131 (GAEGQRPGFGYGG).

Cellular Localization

Myelin membrane; Peripheral membrane protein; Cytoplasmic side (of myelin).

Species Reactivity

Human, bovine and rat, reacts weakly with rabbit. Does not react with guinea pig.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

14-22kDa

Specificity & Comments

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.