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Home >> Antibodies >> MUC5AC (Mucin 5AC / Gastric Mucin)

MUC5AC (Mucin 5AC / Gastric Mucin)

Mouse Monoclonal Antibody [Clone SPM297]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (SPM297).

Product Details

Synonyms

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Positive Control

Human colon or stomach (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Monkey. Rabbit. Cat. Mouse. Rat. Pig. Hedgehog. Chicken. Does not react with Cow.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

>1,000kDa

Specificity & Comments

This MAb recognizes the peptide core of gastric mucin M1 (1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bara J et. al. International Journal of Cancer, 1991, 47(2):304-10.
  2. Bara J et. al. Journal of Immunological Methods, 1992, 149(1):105-13.

Bioinformatics

  •  Entrez Gene ID: 4586
  •  Gene Symbol: MUC5AC
  •  SwissProt: P98088
  •  Chromosome Location: 11p15.5
  •  Unigene: 534332
NBT00031