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Home >> Antibodies >> MUC16 / CA125 (Ovarian Carcinoma Marker)

MUC16 / CA125 (Ovarian Carcinoma Marker)

Mouse Monoclonal Antibody [Clone [5E11]]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC16 Mouse Monoclonal Antibody (5E11). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty University of Porto, Portugal.

Product Details

Synonyms

CA125; Cancer antigen 125; MUC16; Mucin16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125

Positive Control

MDA-MB-468 cells. Ovarian Cancer.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Enzymatically glycosylated fragment of human MUC16 recombinant protein

Cellular Localization

Cell Surface and Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

>2,000kDa

Specificity & Comments

The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs). Membrane-associated and secretory Mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. Epithelial Mucins are large, secreted and cell surface glycoproteins crucial for adhesion modulation, signaling and epithelial cell protection. The number of repeats is highly polymorphic and varies among different alleles. The Mucin family consists of Mucins 1-4, Mucin 5 (AC and B), Mucins 6-8, Mucins 11-13 and Mucins 15-17. The Mucin 16 protein (also commonly referred to as CA125), encoded for by the gene MUC16, is a very high molecular weight tumor antigen consisting of three domains: a carboxy terminal domain, an extracellular domain and an amino terminal domain. Mucin 16, an ovarian cancer-associated antigen, is used as a marker to monitor the progress of epithelial ovarian cancer. It is a hydrophilic membrane-associated protein that may be involved in vitamin A functions.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lara Marcos-Silva, Sara Ricardo, Kowa Chen, Ola Blixt, Emma Arigi, Daniela Pereira, Estrid Hgdall, Ulla Mandel, Eric P. Bennett, Sergey Y. Vakhrushev, Leonor David, Henrik Clausen. A novel monoclonal antibody to a defined peptide epitope in MUC16. Glycobiology (2015) 25 (11): 1172-1182.

Bioinformatics

  •  Entrez Gene ID: 94025
  •  Gene Symbol: CA125
  •  SwissProt: Q8WXI7
  •  Chromosome Location: 19p13.2
  •  Unigene: 432676
NBT02624