Home >> Antibodies >> MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

Mouse Monoclonal Antibody [Clone HMPV]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).

SDS-PAGE Analysis Purified MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV). Confirmation of Integrity and Purity of Antibody

Flow Cytometric Analysis of PFA fixed MCF-7 cells. MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red)

Western Blot Analysis of human MCF-7 cell lysate using MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).

SDS-PAGE Analysis Purified MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV). Confirmation of Integrity and Purity of Antibody

Flow Cytometric Analysis of PFA fixed MCF-7 cells. MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red)

Western Blot Analysis of human MCF-7 cell lysate using MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).

Product Details

Synonyms

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Positive Control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, Immunohistology More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-paraffin) (0.5-1.0ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human breast cancer cell line ZR-75 cells

Cellular Localization

Cytoplasmic and Cell Surface

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

265-400kDa

Specificity & Comments

This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.