Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (GP1.4 + E29)
SDS-PAGE Analysis Purified MUC-1 Mouse Monoclonal Antibody (GP1.4 + E29). Confirmation of Integrity and Purity of Antibody
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions.In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.