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Home >> Antibodies >> MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone MUC1/1887R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).

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SDS-PAGE Analysis of Purified MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).

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Western Blot Analysis of MCF-7 cell lysate using MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).

Product Details

Synonyms

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Positive Control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MUC1 protein

Cellular Localization

Cytoplasmic and cell surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

265-400kDa

Specificity & Comments

This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.

Related Products



200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zotter et. al. Virchows Arch A Pathol Anat Histopathol. 1985;406(2):237-51.
  2. Hilkens et al. Int J Cancer. 1984 Aug 15;34(2):197-206.
  3. Hilkens et al. Eur J Obstet Gynecol Reprod Biol. 1985 Jun;19(6):356-7.

Bioinformatics

  •  Entrez Gene ID: 4582
  •  Gene Symbol: MUC1
  •  SwissProt: P15941
  •  Chromosome Location: 1q22
  •  Unigene: 89603
NBT01887