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Home >> Antibodies >> MTF1

MTF1

Mouse Monoclonal Antibody [Clone MTF1/2649]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MTF1 Mouse Monoclonal Antibody (MTF1/2649).

img

SDS-PAGE Analysis Purified MTF1 Mouse Monoclonal Antibody (MTF1/2649). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Metal regulatory transcription factor 1; Metal response element binding transcription factor 1; Metal responsive transcription factor 1; MRE-binding transcription factor 1; MTF1; Transcription factor MTF1; Zinc regulatory factor (ZRF)

Positive Control

Human kidney.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MTF1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

The metal-responsive element (MRE)-binding transcription factor (MTF-1) stimulates the expression of metallothioneins in response to the exposure of cells to heavy metals. MTF-1 contains six zinc fingers in the DNA binding domain. The phosphorylation of MTF-1 in response to metal exposure appears to play a significant role in the ability of MTF-1 to activate metallothionein transcription. In addition to its role in metallothionein activation, MTF-1 is involved in a post-transcription regulatory complex for ribosomal protein S25. MTF-1, La and p53 inhibit the nuclear export of S25 mRNA in response to nutrient depravation. Furthermore, MTF-1 acts as a chromatin insulator on integrated transgenes in cultured cells to insulate active loci against chromatin silencing.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Adilakshmi, T. and Laine, R.O. 2002. J. Biol. Chem. 277: 4147-4151.

Bioinformatics

  •  Entrez Gene ID: 4520
  •  Gene Symbol: MTF1
  •  SwissProt: Q14872
  •  Chromosome Location: 1p34.3
  •  Unigene: 471991
NBT02649