2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> MTAP (Tumor Suppressor Marker)

MTAP (Tumor Suppressor Marker)

Mouse Monoclonal Antibody [Clone MTAP/1813]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Mouse Monoclonal Antibody (MTAP/1813).

img

SDS-PAGE Analysis Purified MTAP Mouse Monoclonal Antibody (MTAP/1813). Confirmation of Integrity and Purity of Antibody.

img

Western Blot Analysis of Human HeLa, A431, HePG2 and HAP1 cell lysate using MTAP Mouse Monoclonal Antibody (MTAP/1813).

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using MTAP Mouse Monoclonal Antibody (MTAP/1813).

Product Details

Synonyms

BDMF; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAPase; S-methyl-5''-thioadenosine phosphorylase

Positive Control

HepG2, A431, HeLa, HAP1 or MCF-7 cells. Kidney.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

31kDa

Specificity & Comments

Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Nobori, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93: 6203-6208.

Bioinformatics

  •  Entrez Gene ID: 4507
  •  Gene Symbol: MTAP
  •  SwissProt: Q13126
  •  Chromosome Location: 9p21.3
  •  Unigene: 193268
NBT01813