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Home >> Antibodies >> MSH6 (DNA Mismatch Repair Protein)

MSH6 (DNA Mismatch Repair Protein)

Recombinant Rabbit Monoclonal Antibody [Clone MSH6/6654R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Western blot analysis of HCT-116 cell lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R).

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Formalin-fixed, paraffin-embedded human colon from Lynch Disease stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R).

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human esophagus stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R). Inset: PBS instead of primary antibody; secondary only negative control.

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Formalin-fixed, paraffin-embedded dog lymph node stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded cat colon stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein

Positive Control

HCT116, MCF-7 or HeLa cells. Human colon carcinoma or tonsil.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determin

Immunogen

Recombinant fragment (around aa 1-200) of human MSH6 protein (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human. Dog. Cat.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

153kDa

Specificity & Comments

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackwell LJ., et al. J Biol Chem. 1998 Nov 27;273(48):32055-62.
  2. Iaccarino I., et al. EMBO J. 1998 May 1;17(9):2677-86.

Bioinformatics

  •  Entrez Gene ID: 2956
  •  Gene Symbol: MSH6
  •  SwissProt: P52701
  •  Chromosome Location: 2p16.3
  •  Unigene: 445052