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Home >> Antibodies >> MSH2 (DNA Mismatch Repair Marker)

MSH2 (DNA Mismatch Repair Marker)

Mouse Monoclonal Antibody [Clone MSH2/2622]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

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Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

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Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

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Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).

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Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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SDS-PAGE Analysis of Purified MSH2 Mouse Monoclonal Antibody (MSH2/2622). Confirmation of Purity and Integrity of Antibody

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

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Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti-mouse IgG conjugated to CF488 (green). Membrane are stained with Phalloiden (Red).

Product Details

Synonyms

BAT26; COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC1; LCFS2; MSH2; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2

Positive Control

A549 or HepG2 cells. Colon, Thyroid, Testis or Lymph Node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human. Dog

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

100kDa

Specificity & Comments

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Palombo, F., et al. 1994. Mismatch repair and cancer. Nature 367: 417-41

Bioinformatics

  •  Entrez Gene ID: 4436
  •  Gene Symbol: MSH2
  •  SwissProt: IDP43246
  •  Chromosome Location: 2p21-p16.3
  •  Unigene: 597656
NBT02622