Home >> Antibodies >> Moesin

Moesin

Mouse Monoclonal Antibody [Clone MSN/493]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

429.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Western Blot Analysis of human Jurkat cell lysate. Moesin Mouse Monoclonal Antibody (MSN/493).

Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. Moesin Mouse Monoclonal Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Flow Cytometric Analysis of paraformaldehyde-fixed K562 cells. Moesin Mouse Monoclonal Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

SDS-PAGE Analysis Purified Moesin Mouse Monoclonal Antibody (MSN/493). Confirmation of Integrity and Purity of Antibody

Western Blot Analysis of PC3 cell lysate. Moesin Mouse Monoclonal Antibody (MSN/493).

Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Western Blot Analysis of human Jurkat cell lysate. Moesin Mouse Monoclonal Antibody (MSN/493).

Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. Moesin Mouse Monoclonal Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Flow Cytometric Analysis of paraformaldehyde-fixed K562 cells. Moesin Mouse Monoclonal Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

SDS-PAGE Analysis Purified Moesin Mouse Monoclonal Antibody (MSN/493). Confirmation of Integrity and Purity of Antibody

Western Blot Analysis of PC3 cell lysate. Moesin Mouse Monoclonal Antibody (MSN/493).

Product Details

Synonyms

Membrane-organizing extension spike protein; Moesin/anaplastic lymphoma kinase fusion protein; MSN/ALK fusion

Positive Control

K562, HT-29, Jurkat, CH3LC or HUVEC cells. Uterus, placenta, tonsil (both B and T lymphocytes), skeletal muscle, thyroid or kidney.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Moesin protein

Cellular Localization

Cytoplasmic & Cell Surface

Species Reactivity

Human. Does not react with Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

78kDa

Specificity & Comments

Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.