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Home >> Antibodies >> MMP2 / Collagenase Type IV A (Tumor Metastasis Marker)

MMP2 / Collagenase Type IV A (Tumor Metastasis Marker)

Mouse Monoclonal Antibody [Clone 4D3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified MMP2 Mouse Monoclonal Antibody (4D3). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

72kD type IV collagenase; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metalloproteinase-2; MMP2; MONA; Neutrophil gelatinase; PEX; TBE-1

Positive Control

U-138 MG or U-87 MG cells. Human placenta or colon carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MMP2 protein fragment (aa557-569)

Cellular Localization

Cytoplasm. Cell Surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

72kDa (Pro); 63kDa (cleaved)

Specificity & Comments

It recognizes a protein of 72kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Huhtala, P., et al. 1990. Structure of the human type IV collagenase gene. J. Biol. Chem. 265: 11077-11082.

Bioinformatics

  •  Entrez Gene ID: 4313
  •  Gene Symbol: MMP2
  •  SwissProt: P08253
  •  Chromosome Location: 16q12.2
  •  Unigene: 513617