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Home >> Antibodies >> Mitochondrial Marker

Mitochondrial Marker

Mouse Monoclonal Antibody [Clone SPM198]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (SPM198).

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Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (SPM198) labeled in Green (CF488) and Reddot is used to label the nuclei purple.

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Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (SPM198).

Product Details

Synonyms

Not Known

Positive Control

HeLa or HepG2 cells. Hepatic carcinoma.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Semi-Purified mitochondrial preparation

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

MAb 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Epstein, Alan L. and Clevenger, Charles V. In: Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, Ed. CRC Press, Boca Raton, FL, pp. 117-137, 1985.

Bioinformatics

NBT00739