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Home >> Antibodies >> Mitochondria (Marker for Human Cells, Granular RCC s & Salivary Tumor

Mitochondria (Marker for Human Cells, Granular RCC s & Salivary Tumor

Mouse Monoclonal Antibody [Clone 113-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).

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Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (113-1).

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Western Blot analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (113-1).

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Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (113-1) labeled is Green (CF488) and Reddot is used to label the nuclei Red.

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SDS-PAGE Analysis Purified Mitochondria Mouse Monoclonal Antibody (113-1). Confirmation of Integrity and Purity of Antibody.

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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).

Product Details

Synonyms

Not Known

Positive Control

HeLa or HepG2 cells. Hepatocellular carcinoma.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Semi-Purified mitochondrial preparation

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Tickoo SK, Amin MB, Linden MD, Lee MW, Zarbo RJ. Antimitochondrial antibody (113-1) in the differential diagnosis of granular renal cell tumors. Am J Surg Pathol. 1997;21(8):922-30.
  2. Vera-Sempere F, Vera-Sirera B. Usefulness of antimitochondrial antibody 113-1 in diagnosis and classification of salivary gland tumours with oncocytic differentiation. Acta Otorrinolaringol Esp. 2011;62(1):1-9.

Bioinformatics

NBT00739