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Home >> Antibodies >> Milk Fat Globule (Breast Epithelial Marker)

Milk Fat Globule (Breast Epithelial Marker)

Mouse Monoclonal Antibody [Clone MFG-06]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

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Formalin-fixed, paraffin-embedded human Pancreas stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

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Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

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SDS-PAGE Analysis Purified Milk Fat Globule Monoclonal Antibody (MFG-06). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

Breast epithelial antigen BA46; EDIL1; HMFG; Lactadherin; Lactadherin precursor; Medin; MFGE8; MFGM; Milk fat globule-EGF factor 8 (MFG-E8)

Positive Control

Normal breast or breast carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human milk fat globule membrane preparation

Cellular Localization

Cell Surface & Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Larocca D et al. A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains. Cancer Res 1991, 51: 4994-4998
  2. Ceriani RL et al. Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci USA 1977, 74(2):582-6
  3. Corcoran D and Walker RA. Ultrastructural localization of milk fat globule membrane antigens in human breast carcinomas. J Pathol 1990,161(2):161-6

Bioinformatics

  •  Entrez Gene ID: 4240
  •  Gene Symbol: MFGE8
  •  SwissProt: Q08431
  •  Chromosome Location: 15q25
  •  Unigene: 3745
NBT00227