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Home >> Antibodies >> Migfilin-1 / FBLIM1

Migfilin-1 / FBLIM1

Mouse Monoclonal Antibody [Clone FBLIM1/4600]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Migfilin-1 Mouse Monoclonal Antibody (FBLIM1/4600) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with Migfilin-1 Mouse Monoclonal Antibody (FBLIM1/4600) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified Migfilin-1 Mouse Monoclonal Antibody (FBLIM1/4600). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Migfilin-1 Mouse Monoclonal Antibody (FBLIM1/4600)

Product Details

Synonyms

CSX associated LIM (Cal); Filamin binding LIM protein 1 (FBLIM1); Filamin-binding LIM protein 1 (FBLP1); Gt10; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein

Positive Control

A431 or MCF7 cells. Human heart or kidney tissue.

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human FBLIM1 protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

41kDa

Specificity & Comments

Kindlin-2 (also known as Mig-2, Migfilin) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yizeng et al. Cell (2003) 113(1): 37-47.

Bioinformatics

  •  Entrez Gene ID: 54751
  •  Gene Symbol: FBLIM1
  •  SwissProt: Q8WUP2
  •  Chromosome Location: 14q22.1
  •  Unigene: 530101