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Home >> Antibodies >> MIF (Macrophage Migration Inhibitory Factor)

MIF (Macrophage Migration Inhibitory Factor)

Mouse Monoclonal Antibody [Clone MIF/6281]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human prostate stained with MIF Mouse Monoclonal Antibody (MIF/6281). Inset: PBS instead of primary antibody; secondary only negative control.

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Formalin-fixed, paraffin-embedded human prostate stained with MIF Mouse Monoclonal Antibody (MIF/6281). HIER: Tris/EDTA, pH9.0, 45min. 2░: HRP-polymer, 30min. DAB, 5min.

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Western blot analysis of HEK-293 cell lysate using MIF Mouse Monoclonal Antibody (MIF/6281).

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SDS-PAGE Analysis Purified MIF Mouse Monoclonal Antibody (MIF/6281). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MIF-Monospecific Mouse Monoclonal Antibody (MIF/6281).

Product Details

Synonyms

GIF; GLIF; Glycosylation inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MIF protein; MMIF; Phenylpyruvate tautomerase

Positive Control

Human tonsil, thymus or prostate. PC3 or HEK-293 cells.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human MIF protein (exact sequence is proprietary)

Cellular Localization

Secreted. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG

Mol. Weight of Antigen

13kDa

Specificity & Comments

Macrophage migration inhibitory factor, known as MIF or glycosylationinhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor ╬▒ (TNF╬▒), interleukin-1 (IL-1) and interferon-╬│ (IFN-╬│). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Weiser, W.Y., et al. 1989. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc. Natl. Acad. Sci. USA 86:7522-7526.

Bioinformatics

  •  Entrez Gene ID: 4282
  •  Gene Symbol: MIF
  •  SwissProt: P14174
  •  Chromosome Location: 22q11.23
  •  Unigene: 407995