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Home >> Antibodies >> Microphthalmia Transcription Factor (MITF)

Microphthalmia Transcription Factor (MITF)

Mouse Monoclonal Antibody [Clone PCRP-MITF-1D9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified MITF Mouse Monoclonal Antibody (PCRP-MITF-1D9). Confirmation of Integrity and Purity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. MITF Mouse Monoclonal Antibody (PCRP-MITF-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MITF Mouse Monoclonal Antibody (PCRP-MITF-1D9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MITF-Monospecific Mouse Monoclonal Antibody (PCRP-MITF-1D9).

Product Details

Synonyms

BHLHE32; Class E basic helix-loop-helix protein 32 (bHLHe32); CMM8; Mi; Microphthalmia-associated transcription factor; MITF; WS2; WS2A

Positive Control

Jurkat, A-431, HeLa or 501 Mel human melanoma cells.Human melanoma.

Known Applications & Suggested Dilutions

IP, IF, Flow Cyt, Western Blot         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MITF protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

52-56kDa (doublet)

Specificity & Comments

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hemesath P, et. al. MAP kinase links the transcription factor microphthalmia to c-Kit signalling in melanocytes. Nature. 1998, 391:298-301.

Bioinformatics

  •  Entrez Gene ID: 4286
  •  Gene Symbol: MITF
  •  SwissProt: O75030
  •  Chromosome Location: 3p14.1
  •  Unigene: 166017 &618266