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Home >> Antibodies >> Microphthalmia Transcription Factor (MITF)

Microphthalmia Transcription Factor (MITF)

Recombinant Rabbit Monoclonal Antibody [Clone MITF/2987R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R).

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Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R).

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SDS-PAGE Analysis Purified MITF Recombinant Rabbit Monoclonal Antibody (MITF/2987R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

BHLHE32; Class E basic helix-loop-helix protein 32 (bHLHe32); CMM8; Mi; Microphthalmia-associated transcription factor; MITF; WS2; WS2A

Positive Control

Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MiTF protein

Cellular Localization

Nuclear

Species Reactivity

Human. Dog. Does not react with Mouse or Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

52-56kDa (doublet)

Specificity & Comments

MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hemesath P, et. al. 1998, 391:298-301

Bioinformatics

  •  Entrez Gene ID: 4286
  •  Gene Symbol: MITF
  •  SwissProt: O75030
  •  Chromosome Location: 3p14.1
  •  Unigene: 166017, 618266
NBT02987