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Formalin-fixed, paraffin-embedded human stomach (corpus) stained with MGMT Mouse Monoclonal Antibody (MGMT/8128R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human parotid gland stained with MGMT Mouse Monoclonal Antibody (MGMT/8128R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MGMt/8128R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Product Details
Synonyms
Positive Control
Known Applications & Suggested Dilutions
Immunogen
Cellular Localization
Species Reactivity
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross‐links at the O6‐ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6‐position of guanine in DNA (prior to cross‐link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA‐damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
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