Home >> Antibodies >> Melanoma Marker (MART-1 + gp100)

Melanoma Marker (MART-1 + gp100)

Mouse Monoclonal Antibody [Clone DT101+ BC199 + HMB45]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

429.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).

Product Details

Synonyms

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; PMEL17

Positive Control

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

20-22kDa (doublet) (MART-1): 90-100kDa (gp100)

Specificity & Comments

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.